Manufactured by:ELK Biotechnology
RUO

ATRIP (phospho Ser68) rabbit pAb

ATRIP (phospho Ser68) rabbit pAb from ELK Biotechnology

SKU:
ABM109450
Product type
Primary Antibody
TargetATRIP
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemUbiquitous.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

ATRIP (phospho Ser68) rabbit pAb is a research-use antibody from ELK Biotechnology for studying ATRIP in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2012],

Additional antibody information

Specificity Phospho-ATRIP (S68) Polyclonal Antibody detects endogenous levels of ATRIP protein only when phosphorylated at S68.
Validation evidence captions Western Blot analysis of NIH-3T3 cells using Phospho-ATRIP (S68) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ATRIP (Phospho-Ser68) Antibody | Western blot analysis of lysates from NIH/3T3 cells, using ATRIP (Phospho-Ser68) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Fanconi anemia pathway
Also known as
ATRIP; AGS1; ATR-interacting protein; ATM and Rad3-related-interacting protein
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human ATRIP around the phosphorylation site of Ser68. AA range:34-83
Observed band
80kD
Cellular localization
Nucleus . Redistributes to discrete nuclear foci upon DNA damage.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 34-83
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES7732
UniProt entry
Q8WXE1
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00