Bad (phospho Ser134) rabbit pAb from ELK Biotechnology
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Bad (phospho Ser134) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Bad in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008],
| Specificity | Phospho-Bad (S134) Polyclonal Antibody detects endogenous levels of Bad protein only when phosphorylated at S134. |
|---|---|
| Validation evidence captions | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using BAD (Phospho-Ser134) Antibody | Immunofluorescence analysis of HeLa cells, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide. | Immunohistochemistry analysis of paraffin-embedded human brain, using BAD (Phospho-Ser134) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from mouse liver, using BAD (Phospho-Ser134) Antibody. The lane on the right is blocked with the phospho peptide. |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Human | |||||||
| Mouse | |||||||
| Rat |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
BCL2 associated agonist of cell death (BAD) is a pro-apoptotic BH3-only BCL2-family regulator whose activity is strongly controlled by phosphorylation. When active, BAD can engage anti-apoptotic BCL2-family proteins and shift mitochondrial signalling toward intrinsic apoptosis. This page groups ABMIUM catalogue reagents for BAD signalling and cell-death research.
Biological function: BAD is regulated by phosphorylation at multiple serine residues. Survival signalling can promote phosphorylated BAD association with 14-3-3 proteins, limiting its pro-apoptotic activity. Dephosphorylated BAD can bind anti-apoptotic BCL2-family proteins such as BCL-XL and BCL2, favouring activation of the mitochondrial apoptosis programme.
