Manufactured by:ELK Biotechnology
RUO

AMPKγ2 rabbit pAb

AMPKγ2 rabbit pAb from ELK Biotechnology

SKU:
ABM109016
Product type
Primary Antibody
TargetAMPKγ2
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse
Expression systemIsoform B is ubiquitously expressed except in liver and thymus. The highest level is detected in heart with abundant expression in placenta and testis.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

AMPKγ2 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AMPKγ2 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This gene is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jan 2015],

Additional antibody information

Specificity AMPKγ2 Polyclonal Antibody detects endogenous levels of AMPKγ2 protein.
Validation evidence captions Western Blot analysis of K562 using Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Immunofluorescence analysis of A549 cells, using PRKAG2 Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human heart tissue, using PRKAG2 Antibody. The picture on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>FoxO signaling pathway, >>AMPK signaling pathway, >>Longevity regulating pathway, >>Longevity regulating pathway - multiple species, >>Apelin signaling pathway, >>Tight junction, >>Circadian rhythm, >>Thermogenesis, >>Insulin signaling pathway, >>Adipocytokine signaling pathway, >>Oxytocin signaling pathway, >>Glucagon signaling pathway, >>Insulin resistance, >>Non-alcoholic fatty liver disease, >>Alcoholic liver disease, >>Hypertrophic cardiomyopathy
Also known as
PRKAG2; 5'-AMP-activated protein kinase subunit gamma-2; AMPK gamma2; AMPK subunit gamma-2; H91620p
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human PRKAG2. AA range:1-50
Observed band
65kD
Cellular localization
extracellular space,nucleoplasm,cytosol,nucleotide-activated protein kinase complex,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 1-50
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES6523
UniProt entry
Q9UGJ0
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00