Manufactured by:ELK Biotechnology
RUO

Cleaved-Thrombin R (S42) rabbit pAb

Cleaved-Thrombin R (S42) rabbit pAb from ELK Biotechnology

SKU:
ABM108469
Product type
Primary Antibody
TargetThrombin R
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemPlatelets and vascular endothelial cells.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Cleaved-Thrombin R (S42) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Thrombin R in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Coagulation factor II receptor is a 7-transmembrane receptor involved in the regulation of thrombotic response. Proteolytic cleavage leads to the activation of the receptor. F2R is a G-protein coupled receptor family member. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015],

Additional antibody information

Specificity Cleaved-Thrombin R (S42) Polyclonal Antibody detects endogenous levels of fragment of activated Thrombin R protein resulting from cleavage adjacent to S42.
Validation evidence captions Western Blot analysis of Jurkat cells using Cleaved-Thrombin R (S42) Polyclonal Antibody | Western blot analysis of lysates from Jurkat cells, using PAR1 (Cleaved-Ser42) Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Rap1 signaling pathway, >>Calcium signaling pathway, >>cAMP signaling pathway, >>Phospholipase D signaling pathway, >>Neuroactive ligand-receptor interaction, >>PI3K-Akt signaling pathway, >>Complement and coagulation cascades, >>Platelet activation, >>Regulation of actin cytoskeleton, >>Pathogenic Escherichia coli infection, >>Pathways in cancer
Also known as
F2R; CF2R; PAR1; TR; Proteinase-activated receptor 1; PAR-1; Coagulation factor II receptor; Thrombin receptor
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human PAR1. AA range:23-72
Observed band
46kD
Cellular localization
Cell membrane; Multi-pass membrane protein.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 23-72
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES5185
UniProt entry
P25116
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00