Manufactured by:ELK Biotechnology
RUO

CYP11A1 rabbit pAb

CYP11A1 rabbit pAb from ELK Biotechnology

SKU:
ABM108368
Product type
Primary Antibody
TargetCYP11A1
ApplicationsWB, ELISA
Species / reactivityHuman
Expression systemBrain,Choriocarcinoma,Placenta,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

CYP11A1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying CYP11A1 in WB, ELISA workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

cytochrome P450 family 11 subfamily A member 1(CYP11A1) Homo sapiens This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane and catalyzes the conversion of cholesterol to pregnenolone, the first and rate-limiting step in the synthesis of the steroid hormones. Two transcript variants encoding different isoforms have been found for this gene. The cellular location of the smaller isoform is unclear since it lacks the mitochondrial-targeting transit peptide. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity CYP11A1 Polyclonal Antibody detects endogenous levels of CYP11A1 protein.
Validation evidence captions Western Blot analysis of HeLa cells using CYP11A1 Polyclonal Antibody | Western blot analysis of the lysates from HeLa cells using Cytochrome P450 11A1 antibody.
Key facts & specifications
Research areas
>>Steroid hormone biosynthesis, >>Metabolic pathways, >>Ovarian steroidogenesis, >>Aldosterone synthesis and secretion, >>Cortisol synthesis and secretion, >>Cushing syndrome
Also known as
CYP11A1; CYP11A; Cholesterol side-chain cleavage enzyme; mitochondrial; CYPXIA1; Cholesterol desmolase; Cytochrome P450 11A1; Cytochrome P450(scc)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Cytochrome P450 11A1. AA range:412-461
Observed band
60kD
Cellular localization
Mitochondrion inner membrane ; Peripheral membrane protein . Localizes to the matrix side of the mitochondrion inner membrane. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 412-461
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Identifiers & database references
Supplier SKU / catalogue number
ES4947
UniProt entry
P05108
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00