GAL4 DNA Binding Domain rabbit pAb from ELK Biotechnology
Know who made it. See the evidence. Buy through one marketplace.
GAL4 DNA Binding Domain rabbit pAb is a research-use antibody from ELK Biotechnology for studying GAL4 DNA Binding Domain in WB workflows. Specifications and supporting documents below should be reviewed when planning the experiment.
The GAL4 protein of Saccharomyces cerevisiae is one of the most thoroughly characterized transcriptional activators. Since the N-terminal 147 amino acid residues of GAL4 are sufficient to mediate specific and strong binding to DNA, but are incapable of efficient transcriptional activation , this protein fragment has frequently been used to confer specific DNA binding in experiments examining transcriptional activation functions of heterologous proteins. This approach is facilitated by the finding that higher eukaryotes lack endogenous proteins that enhance transcription from the consensus GAL4-binding site. Fusions between GAL4 (aa 1-147) and activating domains from a variety of transcriptional regulatory proteins can activate transcription in yeast, plant, insects and mammalian cells. A unique "two-hybrid" system has been developed using GAL4 fusions in yeast to identify specific protein-protein interactions.
| Specificity | The antibody detects endogenous GAL4 DNA Binding Domain protein. |
|---|---|
| Validation evidence captions | Western blot analysis of Recombinant BD protein using GAL4 DNA Binding Domain Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| GAL4 DNA Binding Domain |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
