Manufactured by:ELK Biotechnology
RUO

Histone H1 (Tri Methyl Lys25) rabbit pAb

Histone H1 (Tri Methyl Lys25) rabbit pAb from ELK Biotechnology

SKU:
ABM106872
Product type
Primary Antibody
TargetHistone H1
ApplicationsWB
Species / reactivityHuman
Expression systemPlacenta,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Histone H1 (Tri Methyl Lys25) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Histone H1 in WB workflows with listed reactivity for Human. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Histones are basic nuclear proteins responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H1 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq, Aug 2015],

Additional antibody information

Specificity The antibody detects endogenous Histone H1 (Tri Methyl Lys25) protein.
Validation evidence captions Western blot analysis of extracts from Hela cells, 1:2000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Key facts & specifications
Research areas
Antibody Research
Also known as
H1K25ME3; HIST1H1A; H1F1; Histone H1.1; Histone H1a; HIST1H1C; H1F2; Histone H1.2; Histone H1c; Histone H1d; Histone H1s-1; HIST1H1D; H1F3; Histone H1.3; Histone H1c; Histone H1s-2; HIST1H1E; H1F4; Histone H1.4; Histone H1b; Histone H1s-4
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB: 1:1000-2000
Immunogen
Synthetic Peptide of Histone H1 (Tri Methyl Lys25)
Observed band
17-25kD
Cellular localization
Nucleus . Chromosome . Mainly localizes in euchromatin.
Assay type
Antibody validation/application
Source organism
Rabbit
Working concentration
WB: 1:1000-2000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using specific immunogen.
Form / buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Identifiers & database references
Supplier SKU / catalogue number
ES20561
UniProt entry
Q02539/P16403/P16402/P10412
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00