Manufactured by:ELK Biotechnology
RUO

Cdc23 rabbit pAb

Cdc23 rabbit pAb from ELK Biotechnology

SKU:
ABM106767
Product type
Primary Antibody
TargetCdc23
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Monkey
Expression systemBone marrow,Brain,Duodenum,Epithelium,Pancreas,Placenta,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Cdc23 rabbit pAb is a research-use antibody from ELK Biotechnology for studying Cdc23 in WB, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene shares strong similarity with Saccharomyces cerevisiae Cdc23, a protein essential for cell cycle progression through the G2/M transition. This protein is a component of anaphase-promoting complex (APC), which is composed of eight protein subunits and highly conserved in eukaryotic cells. APC catalyzes the formation of cyclin B-ubiquitin conjugate that is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. This protein and 3 other members of the APC complex contain the TPR (tetratricopeptide repeat), a protein domain important for protein-protein interaction. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Cdc23 Polyclonal Antibody detects endogenous levels of Cdc23 protein.
Validation evidence captions Western Blot analysis of various cells using Cdc23 Polyclonal Antibody diluted at 1:2000 | Western Blot analysis of COS7 cells using Cdc23 Polyclonal Antibody diluted at 1:2000
Key facts & specifications
Research areas
>>Cell cycle, >>Oocyte meiosis, >>Ubiquitin mediated proteolysis, >>Progesterone-mediated oocyte maturation, >>Human T-cell leukemia virus 1 infection
Also known as
CDC23; ANAPC8; Cell division cycle protein 23 homolog; Anaphase-promoting complex subunit 8; APC8; Cyclosome subunit 8
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human APC8. AA range:251-300
Observed band
69kD
Cellular localization
intracellular,nucleoplasm,anaphase-promoting complex,cytosol,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 251-300
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Monkey
Identifiers & database references
Supplier SKU / catalogue number
ES1925
UniProt entry
Q9UJX2
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00