Manufactured by:ELK Biotechnology
RUO

AQP5 rabbit pAb

AQP5 rabbit pAb from ELK Biotechnology

SKU:
ABM106008
Product type
Primary Antibody
TargetAQP5
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemDetected in skin eccrine sweat glands, at the apical cell membrane and at intercellular canaliculi (at protein level).
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

AQP5 rabbit pAb is a research-use antibody from ELK Biotechnology for studying AQP5 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity AQP5 Polyclonal Antibody detects endogenous levels of AQP5 protein.
Validation evidence captions Western Blot analysis of various cells using AQP5 Polyclonal Antibody | Immunohistochemistry analysis of AQP5 antibody in paraffin-embedded human lung carcinoma tissue. | Western blot analysis of lysate from HUVECcells, using AQP5 antibody.
Key facts & specifications
Research areas
>>Salivary secretion
Also known as
AQP5; Aquaporin-5; AQP-5
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human AQP5. AA range:194-243
Observed band
35kD
Cellular localization
Apical cell membrane ; Multi-pass membrane protein . Cell membrane ; Multi-pass membrane protein . Cytoplasmic vesicle membrane ; Multi-pass membrane protein . Hypotonicity increases location at the cell membrane. Phosphorylation decreases location at the cell membrane. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 194-243
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES1690
UniProt entry
P55064
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

AQP5aquaporin 5

View target guide

AQP5 identifies aquaporin 5 in Homo sapiens (Human). This target record connects NCBI Gene 362, UniProtKB P55064 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
362
UniProtKB
P55064
ELK Biotechnology
£220.00