Manufactured by:ELK Biotechnology
RUO

Smad3 (phospho Ser208) rabbit pAb

Smad3 (phospho Ser208) rabbit pAb from ELK Biotechnology

SKU:
ABM105142
Product type
Primary Antibody
TargetSmad3
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemBrain,Colon carcinoma,Esophagus tumor,Pancreas,Placenta,Spleen,Umbilical cord blood
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Smad3 (phospho Ser208) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Smad3 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009],

Additional antibody information

Specificity Phospho-Smad3 (S208) Polyclonal Antibody detects endogenous levels of Smad3 protein only when phosphorylated at S208.
Validation evidence captions Western Blot analysis of various cells using Phospho-Smad3 (S208) Polyclonal Antibody | Western Blot analysis of Jurkat cells using Phospho-Smad3 (S208) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Smad3 (Phospho-Ser208) Antibody | Immunohistochemistry analysis of paraffin-embedded human colon carcinoma, using Smad3 (Phospho-Ser208) Antibody. The picture on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>FoxO signaling pathway, >>Cell cycle, >>Endocytosis, >>Cellular senescence, >>Wnt signaling pathway, >>TGF-beta signaling pathway, >>Apelin signaling pathway, >>Hippo signaling pathway, >>Adherens junction, >>Signaling pathways regulating pluripotency of stem cells, >>Th17 cell differentiation, >>AGE-RAGE signaling pathway in diabetic complications, >>Hepatitis B, >>Human T-cell leukemia virus 1 infection, >>Pathways in cancer, >>Colorectal cancer, >>Pancreatic cancer, >>Chronic myeloid leukemia, >>Hepatocellular carcinoma, >>Gastric cancer, >>Inflammatory bowel disease, >>Diabetic cardiomyopathy
Also known as
SMAD3; MADH3; Mothers against decapentaplegic homolog 3; MAD homolog 3; Mad3; Mothers against DPP homolog 3; hMAD-3; JV15-2; SMAD family member 3; SMAD 3; Smad3; hSMAD3
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Smad3 around the phosphorylation site of Ser208. AA range:174-223
Observed band
40kD
Cellular localization
Cytoplasm . Nucleus . Cytoplasmic and nuclear in the absence of TGF-beta. On TGF-beta stimulation, migrates to the nucleus when complexed with SMAD4 (PubMed:15799969, PubMed:21145499). Through the action of the phosphatase PPM1A, released from the SMAD2/SMAD4 complex, and exported out of the nucleus by interaction with RANBP1 (PubMed:16751101, PubMed:19289081). Co-localizes with LEMD3 at the nucleus inner membrane (PubMed:15601644). MAPK-mediated phosphorylation appears to have no effect on nuclear import (PubMed:19218245). PDPK1 prevents its nuclear translocation in response to TGF-beta (PubMed:17327236). Localized mainly to the nucleus in the early stages of embryo development with expression becoming evident in the cytoplasm of the inner cell mass at the blastocyst stage (By similarity)
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 174-223
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1493
UniProt entry
P84022
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

SMAD3SMAD family member 3

View target guide

SMAD3 identifies SMAD family member 3 in Homo sapiens (Human). This target record connects NCBI Gene 4088, UniProtKB P84022 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
4088
UniProtKB
P84022
ELK Biotechnology
£220.00