Manufactured by:ELK Biotechnology
RUO

HDAC2 (phospho Ser394) rabbit pAb

HDAC2 (phospho Ser394) rabbit pAb from ELK Biotechnology

SKU:
ABM104824
Product type
Primary Antibody
TargetHDAC2
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat, Monkey
Expression systemWidely expressed; lower levels in brain and lung.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

HDAC2 (phospho Ser394) rabbit pAb is a research-use antibody from ELK Biotechnology for studying HDAC2 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene product belongs to the histone deacetylase family. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3 and H4). This protein forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression and developmental events. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2010],

Additional antibody information

Specificity Phospho-HDAC2 (S394) Polyclonal Antibody detects endogenous levels of HDAC2 protein only when phosphorylated at S394.
Validation evidence captions Western Blot analysis of various cells using Phospho-HDAC2 (S394) Polyclonal Antibody | Western Blot analysis of 3T3 cells using Phospho-HDAC2 (S394) Polyclonal Antibody | Western blot analysis of lysates from LOVO cells, using HDAC2 (Phospho-Ser394) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Cell cycle, >>Longevity regulating pathway - multiple species, >>Notch signaling pathway, >>Neutrophil extracellular trap formation, >>Thyroid hormone signaling pathway, >>Huntington disease, >>Amphetamine addiction, >>Alcoholism, >>Human papillomavirus infection, >>Epstein-Barr virus infection, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Viral carcinogenesis, >>MicroRNAs in cancer, >>Chronic myeloid leukemia
Also known as
HDAC2; Histone deacetylase 2; HD2
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human HDAC2 around the phosphorylation site of Ser394. AA range:360-409
Observed band
55kD
Cellular localization
Nucleus . Cytoplasm .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 360-409
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Monkey
Identifiers & database references
Supplier SKU / catalogue number
ES1417
UniProt entry
Q92769
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

HDAC2histone deacetylase 2

View target guide

HDAC2 identifies histone deacetylase 2 in Homo sapiens (Human). This target record connects NCBI Gene 3066, UniProtKB Q92769 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3066
UniProtKB
Q92769
ELK Biotechnology
£220.00