Manufactured by:ELK Biotechnology
RUO

GATA-1 (phospho Ser310) rabbit pAb

GATA-1 (phospho Ser310) rabbit pAb from ELK Biotechnology

SKU:
ABM104420
Product type
Primary Antibody
TargetGATA-1
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat, Monkey
Expression systemErythrocytes.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

GATA-1 (phospho Ser310) rabbit pAb is a research-use antibody from ELK Biotechnology for studying GATA-1 in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Phospho-GATA-1 (S310) Polyclonal Antibody detects endogenous levels of GATA-1 protein only when phosphorylated at S310.
Validation evidence captions Western Blot analysis of various cells using Phospho-GATA-1 (S310) Polyclonal Antibody diluted at 1:500 cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003,Inventbiotech,MN,USA). | Western blot analysis of lysates from COS7 cells treated with EPO, using GATA1 (Phospho-Ser310) Antibody. The lane on the left is blocked with the phospho peptide.
Key facts & specifications
Research areas
Antibody Research
Also known as
GATA1; ERYF1; GF1; Erythroid transcription factor; Eryf1; GATA-binding factor 1; GATA-1; GF-1; NF-E1 DNA-binding protein
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human GATA1 around the phosphorylation site of Ser310. AA range:277-326
Cellular localization
Nucleus.
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
43kD
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 277-326
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Monkey
Identifiers & database references
Supplier SKU / catalogue number
ES1319
UniProt entry
P15976
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00