Manufactured by:ELK Biotechnology
RUO

HSP70 (Acetyl Lys246) rabbit pAb

HSP70 (Acetyl Lys246) rabbit pAb from ELK Biotechnology

SKU:
ABM103255
Product type
Primary Antibody
TargetHSP70
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemBrain,Cajal-Retzius cell,Embryonic kidney,Epithelium,Fetal
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

HSP70 (Acetyl Lys246) rabbit pAb is a research-use antibody from ELK Biotechnology for studying HSP70 in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Acetyl-HSP70 (K246) Polyclonal Antibody detects endogenous levels of HSP70 protein only when acetylation at K246.
Validation evidence captions Western Blot analysis of rat kidney cells using Acetyl-HSP70 (K246) Polyclonal Antibody. Antibody was diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of RAT-kidney cells using Acetyl-HSP70 (K246) Polyclonal Antibody diluted at 1:1000. Secondary antibody(catalog#:RS0002) was diluted at 1:20000
Key facts & specifications
Research areas
>>Spliceosome, >>MAPK signaling pathway, >>Protein processing in endoplasmic reticulum, >>Endocytosis, >>Longevity regulating pathway - multiple species, >>Antigen processing and presentation, >>Estrogen signaling pathway, >>Prion disease, >>Legionellosis, >>Toxoplasmosis, >>Measles, >>Lipid and atherosclerosis
Also known as
HSPA1A; HSPA1; HSPA1B; Heat shock 70 kDa protein 1A/1B; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
Synthesized acetyl-peptide derived from the Internal region of human HSP70 around the acetylation site of K246.
Observed band
70kD
Cellular localization
Cytoplasm . Nucleus . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Secreted . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES1136
UniProt entry
P0DMV8/P0DMV9
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00