Manufactured by:ELK Biotechnology
RUO

ISG15 rabbit pAb

ISG15 rabbit pAb from ELK Biotechnology

SKU:
ABM103041
Product type
Primary Antibody
TargetISG15
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse
Expression systemDetected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. Expressed in neutrophils, monocytes and lymphocytes. Enhanced expression seen in pancreatic adenocarcinoma, endometrial cancer, and bladder cancer, as compared to non-cancerous tissue. In bladder cancer, the increase in expression exhibits a striking positive correlation with more advanced stages of the disease.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

ISG15 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ISG15 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted. [provided by RefSeq, Dec 2012],

Additional antibody information

Specificity ISG15 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°over night | Immunohistochemical analysis of paraffin-embedded mouse-liver tissue. 1,ISG15 Polyclonal Antibody was diluted at 1:200(4° overnight). 2, EDTA pH 9.0 was used for antigen retrieval(>98°C,20min). 3,Secondary antibody was diluted at 1:200(room temperature, 30min) Negtive control was used by secondary antibody only.)
Key facts & specifications
Research areas
>>RIG-I-like receptor signaling pathway, >>Human papillomavirus infection, >>Epstein-Barr virus infection, >>Coronavirus disease - COVID-19
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
18kD
Cellular localization
Cytoplasm . Secreted . Exists in three distinct states: free within the cell, released into the extracellular space, or conjugated to target proteins.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES11018
UniProt entry
P05161
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ISG15ISG15 ubiquitin like modifier

View target guide

ISG15 identifies ISG15 ubiquitin like modifier in Homo sapiens (Human). This target record connects NCBI Gene 9636, UniProtKB P05161 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
9636
UniProtKB
P05161
ELK Biotechnology
£220.00