ZO1 rabbit pAb 50µL/100µL
Manufactured by:ELK Biotechnology
RUO

ZO1 rabbit pAb

ZO1 rabbit pAb from ELK Biotechnology

SKU:
ABM102666
Product type
Primary Antibody
TargetZO1
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse
Expression systemThe alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

ZO1 rabbit pAb is a research-use antibody from ELK Biotechnology for studying ZO1 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2014],

Additional antibody information

Specificity ZO1 Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Immunofluorescence analysis of A549. 1,primary Antibody(red) was diluted at 1:200(4°C overnight). 2, Goat Anti Rabbit IgG (H&L) - Alexa Fluor 594 Secondary antibody was diluted at 1:1000(room temperature, 50min).3, Picture B: DAPI(blue) 10min.
Key facts & specifications
Research areas
>>Adherens junction, >>Tight junction, >>Gap junction, >>Vibrio cholerae infection, >>Epithelial cell signaling in Helicobacter pylori infection, >>Pathogenic Escherichia coli infection
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000
Immunogen
Synthesized peptide derived from part region of human protein.AA1600-1700
Observed band
192kD
Cellular localization
Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, tight junction . Cell junction . Cell junction, gap junction. Cell projection, podosome . Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact (PubMed:7798316). At podosomal sites, is predominantly localized in the ring structure surrounding the actin core (PubMed:20930113). Colocalizes with SPEF1 at sites of cell-cell contact in intestinal epithelial cells (PubMed:31473225). .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 IF 1:100-300 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES10356
UniProt entry
Q07157
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00