Manufactured by:ELK Biotechnology
RUO

Recombinant Mouse EPO (C-6His)

Recombinant Mouse EPO (C-6His) from ELK Biotechnology

SKU:
ABM100853
Product type
Recombinant Proteins
TargetEPO
ApplicationsFunctional assay
Purity>95% by SDS-PAGE

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Recombinant Mouse EPO (C-6His) is a research-use recombinant protein from ELK Biotechnology preparation representing EPO supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.

Description

Recombinant Mouse Erythropoietin is produced by our Mammalian expression system and the target gene encoding Ala27-Arg192 is expressed with a 6His tag at the C-terminus.

Background

Erythropoietin (EPO) is a glycoprotein hormone that is principally known for its role in erythropoiesis, where it is responsible for stimulating proliferation and differentiation of erythroid progenitor cells. Erythropoietin is a member of the EPO/TPO family. It is a secreted, glycosylated cytokine composed of four alpha helical bundles. The differentiation of CFU-E (Colony Forming Unit-Erythroid) cells into erythrocytes can only be accomplished in the presence of EPO. Physiological levels of EPO in adult mammals are maintained primarily by the kidneys, whereas levels in fetal or neonatal mammals are maintained by the liver. EPO also can exert various non-hematopoietic activities, including vascularization and proliferation of smooth muscle, neural protection during hypoxia, and stimulation of certain B cells. Genetic variation in erythropoietin is associated with susceptbility to microvascular complications of diabetes type 2. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy.

Handling information

Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Delivery condition The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Key facts & specifications
Research areas
Molecular Biology
Also known as
Erythropoietin; Epoetin; EPO
Assay type
Recombinant protein
Molecular weight
19.4 KDa
Form / buffer
Lyophilized
Endotoxin level
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Quantity per pack
10ug
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpecificationPurityEndotoxinBioactivity
Product QC
Identifiers & database references
Supplier SKU / catalogue number
EPT129
UniProt entry
Q0VED9
Storage, stability & shipping
Storage conditions
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

EPOerythropoietin

View target guide

EPO identifies erythropoietin in Homo sapiens (Human). This target record connects NCBI Gene 2056, UniProtKB P01588 and related ABMIUM catalogue products.

Biological function: EPO is a secreted glycoprotein hormone that regulates erythrocyte proliferation and differentiation and helps maintain circulating red-cell mass. Binding to EPOR activates receptor-associated JAK2 and downstream signalling.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
2056
UniProtKB
P01588
ELK Biotechnology
£337.00