Manufactured by:ELK Biotechnology
RUO

Recombinant Human AFP (C-6His)

Recombinant Human AFP (C-6His) from ELK Biotechnology

SKU:
ABM100833
Product type
Recombinant Proteins
TargetAFP
ApplicationsProtein research
Purity>95% by SDS-PAGE

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Recombinant Human AFP (C-6His) is a research-use recombinant protein from ELK Biotechnology preparation representing AFP supplied as Lyophilized. Review the expression, purity, formulation and storage specifications below before selecting it for an assay or functional workflow.

Description

Recombinant Human Alpha-fetoprotein is produced by our Mammalian expression system and the target gene encoding Arg19-Val609 is expressed with a 6His tag at the C-terminus.

Background

Alpha-fetoprotein (AFP) is classified as a member of the albuminoid gene superfamily consisting of albumin, AFP, vitaminD (Gc) protein, and alpha-albumin. AFP is a glycoprotein of 591 amino acids and a carbohydrate moiety. AFP is a major plasma protein produced by the yolk sac and the liver during fetal development. It is thought to be the fetal form of serum albumin. AFP binds to copper, nickel, fatty acids and bilirubin and is found in monomeric, dimeric and trimeric forms. AFP is one of the several embryo-specific proteins and is adominant serum protein as early in human embryonic life as one month, when albumin and transferrin are present in relatively small amounts. It is first synthesized in the human by the yolk sac and liver (1-2 months) and subsequently predominantly in the liver. A small amount of AFP is produced by the GI tract of the human conceptus. It has been proved that AFP may reappear in the serum in elevated amounts in adult life in association with normal restorative processes and with malignnt growth. Alpha-fetoprotein (AFP) is a specific marker for hepatocellular carcinoma (HCC), teratoblastomas, and neural tube defect (NTD).

Handling information

Reconstitution Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100µg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
Delivery condition The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below.
Key facts & specifications
Research areas
Molecular Biology
Also known as
Alpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin; AFP; HPAFP
Assay type
Recombinant protein
Molecular weight
67.3 KDa
Form / buffer
Lyophilized
Endotoxin level
Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
Quantity per pack
50ug
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpecificationPurityEndotoxinBioactivity
Product QC
Identifiers & database references
Supplier SKU / catalogue number
EPT109
UniProt entry
P02771
Storage, stability & shipping
Storage conditions
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Long-term storage
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

AFPalpha fetoprotein

View target guide

AFP (alpha fetoprotein; alpha-fetoprotein) encodes a major fetal plasma protein produced predominantly by the yolk sac and fetal liver. An albumin-family protein, AFP binds fatty acids, bilirubin and several metal ions and is widely studied in fetal development and liver biology.

Biological function: Alpha-fetoprotein (AFP) is a secreted fetal plasma protein structurally related to serum albumin. It binds fatty acids, bilirubin, copper and nickel and functions as a carrier protein during fetal life, with high production by the yolk sac and fetal liver before expression falls after birth.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
174
UniProtKB
P02771
ELK Biotechnology
£523.00