Manufactured by:ELK Biotechnology
RUO

ATG5 (3C7) Mouse mAb

ATG5 (3C7) Mouse mAb from ELK Biotechnology

SKU:
ABM102463
Product type
Primary Antibody
TargetATG5 (3C7)
ApplicationsWB, IHC
Species / reactivityHuman, Mouse, Rat

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

ATG5 (3C7) Mouse mAb is a research-use antibody from ELK Biotechnology for studying ATG5 (3C7) in WB, IHC workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Autophagy is generally activated by conditions of nutrient deprivation but has also been associated with a number of physiological processes including development, differentiation, neurodegeneration, infection, and cancer. Formation of the autophagosome involves a ubiquitin-like conjugation system in which Atg12 is covalently bound to Atg5 and targeted to autophagosome vesicles. This conjugation reaction is mediated by the ubiquitin E1-like enzyme Atg7 and the E2-like enzyme Atg10.

Additional antibody information

Validation evidence captions Immunohistochemical analysis of paraffin-embedded Human Ovarian Carcinoma Tissue using ATG5 (EM1374)Mouse mAb diluted at 1:200. | Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using ATG5 (EM1374)Mouse mAb diluted at 1:200. | Western blot analysis of Hela Cell Lysate using ATG5 (EM1374)Mouse mAb diluted at 1:10000.
Key facts & specifications
Research areas
Antibody Research
Also known as
APG5, APG5L, ASP, Autophagy protein 5 antibody
Host species
Mouse
Clonality
Monoclonal
Isotype
IgG1
Dilution range
WB 1:5,000-10,000 IHC 1:100-200
Immunogen
Recombinant Protein
Observed band
55kDa
Cellular localization
Cytoplasm, Membrane
Assay type
Antibody validation/application
Source organism
Mouse
Concentration
1mg/mL
Working concentration
WB 1:5,000-10,000 IHC 1:100-200
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
EM1374
UniProt entry
Q9H1Y0
Storage, stability & shipping
Storage conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Long-term storage
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Storage warning
Avoid repeated freeze-thaw cycles.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00