Manufactured by:ELK Biotechnology
RUO

Human PAK1(p21 activated kinase1) Microsample ELISA Kit

Human PAK1(p21 activated kinase1) Microsample ELISA Kit from ELK Biotechnology

SKU:
ABM80762
Product type
ELISA Kits
TargetPAK1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Other biological fluids

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human PAK1(p21 activated kinase1) Microsample ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring PAK1 in Serum, Plasma, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PAK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PAK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PAK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PAK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include Alpha PAK; p21 activated kinase 1; p65 PAK; PAK 1; PAK1; PAKalpha.

Key facts & specifications
Research areas
Enzyme & Kinase, Metabolic pathway
Also known as
Alpha PAK; p21 activated kinase 1; p65 PAK; PAK 1; PAK1; PAKalpha
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
6 pg/mL
Detection range
15.63-1000 pg/mL
Plate format
96-well pre-coated
Form / buffer
Pre-coated microplate kit
Quantity per pack
48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSerumPlasmaOther biological fluids
Sample compatibility
Identifiers & database references
Supplier SKU / catalogue number
ELK8847MS
UniProt entry
Q13153
Storage, stability & shipping
Storage conditions
Store according to the conditions stated in the product manual.
Long-term storage
Store according to the conditions stated in the product manual.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PAK1p21 (RAC1) activated kinase 1

View target guide

PAK1 identifies p21 (RAC1) activated kinase 1 in Homo sapiens (Human). This target record connects NCBI Gene 5058, UniProtKB Q13153 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5058
UniProtKB
Q13153
ELK Biotechnology
£284.00