Manufactured by:ELK Biotechnology
RUO

Cattle TGFb1(Transforming Growth Factor Beta 1) ELISA Kit

Cattle TGFb1(Transforming Growth Factor Beta 1) ELISA Kit from ELK Biotechnology

SKU:
ABM70196
Product type
ELISA Kits
TargetTGFb1
ApplicationsELISA
Species / reactivityCattle
Sample typeSerum, Platelet-poor plasma, Cell culture supernatant, Other biological fluids

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Cattle TGFb1(Transforming Growth Factor Beta 1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring TGFb1 in Serum, Platelet-poor plasma, Cell culture supernatant, Other biological fluids samples from Cattle. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle TGFb1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle TGFb1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle TGFb1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide.

Key facts & specifications
Research areas
Cytokine, Tumor immunity, Infection immunity
Also known as
TGF Beta 1; TGF-B1; CED; DPD1; LAP; Camurati-Engelmann Disease; Latency-associated peptide
Assay type
Competitive Inhibition
Detection method
Colorimetric (TMB/HRP)
Sensitivity
4.8 pg/mL
Detection range
15.63-1000 pg/mL
Plate format
96-well pre-coated
Form / buffer
Pre-coated microplate kit
Quantity per pack
48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSerumPlatelet-poor plasmaCell culture supernatantsOther biological fluids
Sample compatibility
Identifiers & database references
Supplier SKU / catalogue number
ELK5467
UniProt entry
P18341
Storage, stability & shipping
Storage conditions
Store according to the conditions stated in the product manual.
Long-term storage
Store according to the conditions stated in the product manual.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

TGFB1transforming growth factor beta 1

View target guide

TGFB1 identifies transforming growth factor beta 1 in Homo sapiens (Human). This target record connects NCBI Gene 7040, UniProtKB P01137 and related ABMIUM catalogue products.

Biological function: TGFB1 is produced as a latent precursor complex. Regulated activation releases TGF-beta-1, a signalling cytokine with broad roles in cell growth, differentiation, extracellular-matrix regulation and immune responses.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
7040
UniProtKB
P01137
ELK Biotechnology
£366.00