Manufactured by:ELK Biotechnology
RUO

A20 rabbit pAb

A20 rabbit pAb from ELK Biotechnology

SKU:
ABM105501
Product type
Primary Antibody
TargetA20
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse
Expression systemEpithelium,Testis,Trachea,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

A20 rabbit pAb is a research-use antibody from ELK Biotechnology for studying A20 in WB, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene was identified as a gene whose expression is rapidly induced by the tumor necrosis factor (TNF). The protein encoded by this gene is a zinc finger protein and ubiqitin-editing enzyme, and has been shown to inhibit NF-kappa B activation as well as TNF-mediated apoptosis. The encoded protein, which has both ubiquitin ligase and deubiquitinase activities, is involved in the cytokine-mediated immune and inflammatory responses. Several transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2012],

Additional antibody information

Specificity A20 Polyclonal Antibody detects endogenous levels of A20 protein.
Validation evidence captions Western Blot analysis of various cells using A20 Polyclonal Antibody | Western Blot analysis of 293 cells using A20 Polyclonal Antibody | Immunofluorescence analysis of HepG2 cells, using TNAP3 Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from RAW264.7 cells, using TNAP3 Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>NF-kappa B signaling pathway, >>Necroptosis, >>NOD-like receptor signaling pathway, >>IL-17 signaling pathway, >>TNF signaling pathway, >>Measles, >>Epstein-Barr virus infection
Also known as
TNFAIP3; OTUD7C; Tumor necrosis factor alpha-induced protein 3; TNF alpha-induced protein 3; OTU domain-containing protein 7C; Putative DNA-binding protein A20; Zinc finger protein A20
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human TNAP3. AA range:321-370
Observed band
89kD
Cellular localization
Cytoplasm. Nucleus. Lysosome.; [A20p50]: Cytoplasm.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 321-370
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES1556
UniProt entry
P21580
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00