Rat FGF21(Fibroblast Growth Factor 21) ELISA Kit from ELK Biotechnology
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Rat FGF21(Fibroblast Growth Factor 21) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring FGF21 in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat FGF21. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat FGF21. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat FGF21, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat FGF21 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include Fgf21; FGF-21.
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Serum | Plasma | Tissue homogenates | Cell lysates | Cell culture supernatants | Other biological fluids |
|---|---|---|---|---|---|---|
| Sample compatibility |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
Fibroblast growth factor 21 (FGF21) is a secreted endocrine member of the FGF family and an important metabolic regulator. This ABMIUM record links human FGF21 (NCBI Gene 26291; UniProt Q9NSA1) to catalogue-backed research products.
Biological function: Fibroblast growth factor 21 (FGF21) acts as a secreted endocrine metabolic regulator. Curated human records describe stimulation of glucose uptake in adipose tissue and broader roles in metabolic regulation.
