4E-BP1 (phospho Thr69) rabbit pAb from ELK Biotechnology
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4E-BP1 (phospho Thr69) rabbit pAb is a research-use antibody from ELK Biotechnology for studying 4E-BP1 in WB, IHC, IF, ELISA workflows with listed reactivity for Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.
EIF4EBP1 encodes one member of a family of translation repressor proteins. The eukaryotic translation initiation factor 4E binding protein 1 directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
| Specificity | Phospho-4E-BP1 (T69) Polyclonal Antibody detects endogenous levels of 4E-BP1 protein only when phosphorylated at T69. |
|---|---|
| Validation evidence captions | Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using 4E-BP1 (Phospho-Thr69) Antibody | Immunohistochemistry analysis of paraffin-embedded human brain carcinoma, using 4E-BP1 (Phospho-Thr69) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using 4E-BP1 (Phospho-Thr69) Antibody. The lane on the right is blocked with the phospho peptide. |
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Evidence status by application and model.
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Mouse | |||||||
| Rat |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
