Manufactured by:Elabscience
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Human AIRE (Autoimmune Regulator) CLIA Kit

Human AIRE (Autoimmune Regulator) CLIA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM10938-1
Product type
Assay Kits
TargetAIRE

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human AIRE (Autoimmune Regulator) CLIA Kit from Elabscience is intended for research workflows involving AIRE. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Research areas
Cancer, Epigenetics and Nuclear Signaling, Immunology
Cross-reactivity
This kit recognizes Human AIRE in samples.No significant cross-reactivity or interference between Human AIRE and analogues was observed
Incubation temperature
2-8℃/-20℃
Assay type
CLIA Kits
Detection method
Chemiluminescence
Plate format
48-well microplate plates pre-coated with anti-AIRE capture antibody
Technical note
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AIRE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AIRE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AIRE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AIRE. You can calculate the concentration of Human AIRE in the samples by comparing the RLU value of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-CL-H0428_48T
Gene symbol
AIRE
Gene ID
326
UniProt accession
O43918
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

AIREautoimmune regulator

View target guide

AIRE (autoimmune regulator) is a transcriptional regulator central to thymic immune tolerance, where it promotes expression of tissue-restricted antigens that support deletion of autoreactive T cells.

Biological function: AIRE regulates transcriptional programs in thymic epithelial cells and helps expose developing T cells to tissue-restricted self-antigens. Authoritative NCBI and UniProt evidence supports its chromatin-associated role in central self-tolerance and negative selection of autoreactive T cells.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
326
UniProtKB
O43918
Elabscience
£407.00£325.60