Dual Luciferase Reporter Gene Luminescence Assay Kit (Glow Type)

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Manufactured by:Elabscience
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Dual Luciferase Reporter Gene Luminescence Assay Kit (Glow Type)

Dual Luciferase Reporter Gene Luminescence Assay Kit (Glow Type) with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM10326-1
Product type
Assay Kits

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Dual Luciferase Reporter Gene Luminescence Assay Kit (Glow Type) from Elabscience is intended for research workflows. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Research areas
Amino Acids and Proteins
Incubation temperature
This product can be stored at -20°C for 12 months with shading light.
Assay type
Assay Kits
Detection method
Fluorometric method
Technical note
Dual luciferase reporter gene is used to detect the luminescence reaction produced by luciferase catalyzed substrates. The detection system includes firefly luciferase reporter gene and renilla luciferase reporter gene. As an internal parameter of transfection, the renilla luciferase can normalize the experimental data. Firefly luciferase reporter gene refers to a reporting system that uses fluorescein as substrate to detect fluorescein luciferase activity. The reaction can occur in the presence of ATP, magnesium ions and oxygen, resulting in yellow-green fluorescence signals. The advantage of the reporter gene is that the expression product of the reporter gene has detectable enzyme activity as soon as the translation is completed. Renilla luciferase reporter gene refers to a reporting system that uses coelenterazine as substrate to detect the activity of renilla luciferase. This reaction does not depend on ATP to participate in the enzymatic reaction. During the reaction, a blue fluorescence signal is generated.
The reaction principle of this kit is that in the presence of oxygen, ATP and magnesium ions at the same time, firefly luciferin and coelenterazine are catalyzed and oxidized successively by firefly luciferase and renilla luciferase in the samples to produce different fluorescence signals, and the expression levels of firefly luciferase and renilla luciferase in the samples can be detected by chemiluminescence immunoassay analyzer.
Identifiers & database references
Supplier SKU / catalogue number
E-BC-F074_48T
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Elabscience
£112.00£89.60