Manufactured by:ELK Biotechnology
RUO

Glycogen Synthase 1 (phospho Ser645) rabbit pAb

Glycogen Synthase 1 (phospho Ser645) rabbit pAb from ELK Biotechnology

SKU:
ABM108691
Product type
Primary Antibody
TargetGlycogen Synthase 1
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemEndometrium,Heart,Kidney,Lymph,Muscle,Skin,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Glycogen Synthase 1 (phospho Ser645) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Glycogen Synthase 1 in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene catalyzes the addition of glucose monomers to the growing glycogen molecule through the formation of alpha-1,4-glycoside linkages. Mutations in this gene are associated with muscle glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009],

Additional antibody information

Specificity Phospho-Glycogen Synthase 1 (S645) Polyclonal Antibody detects endogenous levels of Glycogen Synthase 1 protein only when phosphorylated at S645.
Validation evidence captions Western Blot analysis of 293 cells using Phospho-Glycogen Synthase 1 (S645) Polyclonal Antibody | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Glycogen Synthase (Phospho-Ser645) Antibody | Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Glycogen Synthase (Phospho-Ser645) Antibody. The picture on the right is blocked with the phospho peptide. | Western blot analysis of lysates from NIH/3T3 cells treated with PMA 125ng/ml 30', using Glycogen Synthase (Phospho-Ser645) Antibody. The lane on the right is blocked with the phospho peptide.
Key facts & specifications
Research areas
>>Starch and sucrose metabolism, >>Metabolic pathways, >>PI3K-Akt signaling pathway, >>AMPK signaling pathway, >>Insulin signaling pathway, >>Glucagon signaling pathway, >>Insulin resistance, >>Diabetic cardiomyopathy
Also known as
GYS1; GYS; Glycogen [starch] synthase; muscle
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Glycogen Synthase around the phosphorylation site of Ser645. AA range:611-660
Observed band
83kD
Cellular localization
cytosol,membrane,inclusion body,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 611-660
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES5702
UniProt entry
P13807
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00