Manufactured by:ELK Biotechnology
RUO

Cleaved-GGT4 HC (T472) rabbit pAb

Cleaved-GGT4 HC (T472) rabbit pAb from ELK Biotechnology

SKU:
ABM108611
Product type
Primary Antibody
TargetGGT4 HC
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemWidely expressed, but at low level, except in the airway epithelial cells. Detected in brain, heart, kidney, liver, lung, spleen, testis and trachea.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Cleaved-GGT4 HC (T472) rabbit pAb is a research-use antibody from ELK Biotechnology for studying GGT4 HC in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene is a member of a gene family that encodes enzymes involved in both the metabolism of glutathione and in the transpeptidation of amino acids. Changes in the activity of gamma-glutamyltransferase may signal preneoplastic or toxic conditions in the liver or kidney. The protein encoded by this gene consists of a heavy and a light chain, and it can interact with CT120, a plasma membrane-associated protein that is possibly involved in lung carcinogenesis. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Cleaved-GGT4 HC (T472) Polyclonal Antibody detects endogenous levels of fragment of activated GGT4 HC protein resulting from cleavage adjacent to T472.
Validation evidence captions Western blot analysis of lysates from Jurkat cells, treated with etoposide 25uM 24h, using Gamma-glutamyltransferase 4 (heavy chain,Cleaved-Thr472) Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Taurine and hypotaurine metabolism, >>Glutathione metabolism, >>Metabolic pathways
Also known as
GGT7; GGTL3; GGTL5; Gamma-glutamyltransferase 7; GGT 7; Gamma-glutamyltransferase-like 3; Gamma-glutamyltransferase-like 5; Gamma-glutamyltranspeptidase 7; Glutathione hydrolase 7
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Gamma-glutamyltransferase 4. AA range:423-472
Observed band
50kD
Cellular localization
Membrane ; Single-pass type II membrane protein .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 423-472
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES5516
UniProt entry
Q9UJ14
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00