Manufactured by:ELK Biotechnology
RUO

PFKM rabbit pAb

PFKM rabbit pAb from ELK Biotechnology

SKU:
ABM107419
Product type
Primary Antibody
TargetPFKM
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemBrain,Liver,Muscle,Skeletal muscle,Thymus,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

PFKM rabbit pAb is a research-use antibody from ELK Biotechnology for studying PFKM in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009],

Additional antibody information

Specificity PFKM Polyclonal Antibody detects endogenous levels of PFKM protein.
Validation evidence captions Western Blot analysis of various cells using PFKM Polyclonal Antibody | Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunohistochemistry analysis of PFK-1 antibody in paraffin-embedded human brain tissue. | Western blot analysis of lysate from Hela cells, using PFK-1 antibody.
Key facts & specifications
Research areas
>>Glycolysis / Gluconeogenesis, >>Pentose phosphate pathway, >>Fructose and mannose metabolism, >>Galactose metabolism, >>Metabolic pathways, >>Carbon metabolism, >>Biosynthesis of amino acids, >>RNA degradation, >>HIF-1 signaling pathway, >>AMPK signaling pathway, >>Thyroid hormone signaling pathway, >>Glucagon signaling pathway, >>Central carbon metabolism in cancer
Also known as
PFKM; PFKX; 6-phosphofructokinase; muscle type; Phosphofructo-1-kinase isozyme A; PFK-A; Phosphofructokinase-M; Phosphofructokinase 1; Phosphohexokinase
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human PFK-1. AA range:320-369
Observed band
85kD
Cellular localization
Cytoplasm .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 320-369
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES3181
UniProt entry
P08237
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

PFKMphosphofructokinase, muscle

View target guide

PFKM identifies phosphofructokinase, muscle in Homo sapiens (Human). This target record connects NCBI Gene 5213, UniProtKB P08237 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
5213
UniProtKB
P08237
ELK Biotechnology
£220.00