Manufactured by:ELK Biotechnology
RUO

HCCS rabbit pAb

HCCS rabbit pAb from ELK Biotechnology

SKU:
ABM107124
Product type
Primary Antibody
TargetHCCS
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Mouse, Monkey
Expression systemBrain,Liver,Ovary,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

HCCS rabbit pAb is a research-use antibody from ELK Biotechnology for studying HCCS in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

holocytochrome c synthase(HCCS) Homo sapiens The protein encoded by this gene is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Defects in this gene are a cause of microphthalmia syndromic type 7 (MCOPS7). Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2010],

Additional antibody information

Specificity HCCS Polyclonal Antibody detects endogenous levels of HCCS protein.
Validation evidence captions Western Blot analysis of various cells using HCCS Polyclonal Antibody diluted at 1:2000 | Immunofluorescence analysis of MCF7 cells, using Cytochrome c-type Heme Lyase Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human tonsil tissue, using Cytochrome c-type Heme Lyase Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from HeLa, HepG2, and COLO cells, using Cytochrome c-type Heme Lyase Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Porphyrin metabolism, >>Metabolic pathways
Also known as
HCCS; CCHL; Cytochrome c-type heme lyase; CCHL; Holocytochrome c-type synthase
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human Cytochrome c-type Heme Lyase. AA range:81-130
Observed band
31kD
Cellular localization
Mitochondrion inner membrane . Membrane ; Lipid-anchor .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 81-130
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Monkey
Identifiers & database references
Supplier SKU / catalogue number
ES2496
UniProt entry
P53701
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00