Manufactured by:ELK Biotechnology
RUO

Fucokinase rabbit pAb

Fucokinase rabbit pAb from ELK Biotechnology

SKU:
ABM107070
Product type
Primary Antibody
TargetFucokinase
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse, Monkey
Expression systemExpressed in fibroblasts.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Fucokinase rabbit pAb is a research-use antibody from ELK Biotechnology for studying Fucokinase in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

fucokinase(FUK) Homo sapiens The protein encoded by this gene belongs to the GHMP (galacto-, homoserine, mevalonate and phosphomevalonate) kinase family and catalyzes the phosphorylation of L-fucose to form beta-L-fucose 1-phosphate. This enzyme catalyzes the first step in the utilization of free L-fucose in glycoprotein and glycolipid synthesis. L-fucose may be important in mediating a number of cell-cell interactions such as blood group antigen recognition, inflammation, and metastatis. While several transcript variants may exist for this gene, the full-length nature of only one has been described to date. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity Fucokinase Polyclonal Antibody detects endogenous levels of Fucokinase protein.
Validation evidence captions Western Blot analysis of various cells using Fucokinase Polyclonal Antibody | Immunofluorescence analysis of NIH/3T3 cells, using FUK Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COS7 cells, treated with insulin 0.01U/ml 45', using FUK Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
>>Fructose and mannose metabolism, >>Amino sugar and nucleotide sugar metabolism, >>Metabolic pathways, >>Biosynthesis of nucleotide sugars
Also known as
FUK; L-fucose kinase; Fucokinase
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human FUK. AA range:11-60
Observed band
110kD
Cellular localization
cytoplasm,cytosol,
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 11-60
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Monkey
Identifiers & database references
Supplier SKU / catalogue number
ES2379
UniProt entry
Q8N0W3
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00