Rac1/CDC42 (Phospho Ser71) rabbit pAb

Rac1/CDC42 (Phospho Ser71) rabbit pAb

Rac1/CDC42 (Phospho Ser71) rabbit pAb
Manufactured by:ELK Biotechnology
RUO

Rac1/CDC42 (Phospho Ser71) rabbit pAb

Rac1/CDC42 (Phospho Ser71) rabbit pAb from ELK Biotechnology

SKU:
ABM106825
Product type
Primary Antibody
TargetRac1/CDC42
ApplicationsWB, IHC, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemIsoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
PurityThe antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Rac1/CDC42 (Phospho Ser71) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Rac1/CDC42 in WB, IHC, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Additional antibody information

Specificity This antibody detects endogenous levels of Human,Mouse,Rat Rac1/CDC42 (Phospho Ser71)
Validation evidence captions Western blot analysis of Rac1(Phospho-Ser71) antibody in 293 cells lysates, cell treated or untreated with PMA 125ng/ml 30', 4° over night, secondary antibody(cat: RS0002 was diluted at 1:10000, 37° 1hour. | Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Rac1(Phospho-Ser71) antibody | Immunohistochemical analysis of paraffin-embedded human uterus. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min). | Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200(4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).
Key facts & specifications
Research areas
>>MAPK signaling pathway, >>Ras signaling pathway, >>Rap1 signaling pathway, >>cAMP signaling pathway, >>Chemokine signaling pathway, >>Sphingolipid signaling pathway, >>Phagosome, >>PI3K-Akt signaling pathway, >>Wnt signaling pathway, >>Axon guidance, >>VEGF signaling pathway, >>Osteoclast differentiation, >>Focal adhesion, >>Adherens junction, >>Tight junction, >>Neutrophil extracellular trap formation, >>Toll-like receptor signaling pathway, >>Natural killer cell mediated cytotoxicity, >>B cell receptor signaling pathway, >>Fc epsilon RI signaling pathway, >>Fc gamma R-mediated phagocytosis, >>Leukocyte transendothelial migration, >>Neurotrophin signaling pathway, >>Regulation of actin cytoskeleton, >>Non-alcoholic fatty liver disease, >>AGE-RAGE signaling pathway in diabetic complications, >>Pancreatic secretion, >>Amyotrophic lateral sclerosis, >>Prion disease, >>Pathways of neurodegeneration - multiple diseases, >>Bacterial invasion of epithelial cells, >>Epithelial cell signaling in Helicobacter pylori infect
Also known as
Ras-related C3 botulinum toxin substrate 1 (Cell migration-inducing gene 5 protein;Ras-like protein TC25;p21-Rac1)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Immunogen
Synthesized peptide derived from human Rac1/CDC42 (Phospho Ser71)
Cellular localization
Cell membrane ; Lipid-anchor ; Cytoplasmic side . Melanosome . Cytoplasm . Cell projection, lamellipodium . Cell projection, dendrite . Cell junction, synapse . Nucleus . Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (PubMed:1903399). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Localizes to the lamellipodium in a SH3RF1-dependent manner (By similarity). In macrophages, cytoplasmic location increases upon CSF1 stimulation (By similarity). Activation by GTP-binding promotes nuclear localization (PubMed:12551911). .
Assay type
Antibody validation/application
Source organism
Rabbit
Molecular weight
21kD
Concentration
1 mg/ml
Working concentration
WB 1:500-2000;IHC-p 1:50-300; ELISA 2000-20000
Purification
The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES20202
UniProt entry
P63000/P15153/P60763/P60953
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00