Manufactured by:ELK Biotechnology
RUO

Arylsulfatase I rabbit pAb

Arylsulfatase I rabbit pAb from ELK Biotechnology

SKU:
ABM106109
Product type
Primary Antibody
TargetArylsulfatase I
ApplicationsWB, IHC, IF, ELISA
Species / reactivityHuman, Monkey
Expression systemExpressed in placenta, in embryonic stem cells, fetal eyes and lens.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Arylsulfatase I rabbit pAb is a research-use antibody from ELK Biotechnology for studying Arylsulfatase I in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a protein that belongs to a large family of sulfatases that hydrolyze sulfate esters and sulfamates. Members of this family play a role in several cellular processes, including hormone synthesis, cell signaling in development and degradation of macromolecules. The protein encoded by this gene is thought to be secreted, and to function in extracellular space. [provided by RefSeq, Jul 2016],

Additional antibody information

Specificity Arylsulfatase I Polyclonal Antibody detects endogenous levels of Arylsulfatase I protein.
Validation evidence captions Western Blot analysis of various cells using Arylsulfatase I Polyclonal Antibody | Immunohistochemical analysis of paraffin-embedded Human brain. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide. | Immunofluorescence analysis of MCF7 cells, using ARSI Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from COS7 cells, using ARSI Antibody. The lane on the right is blocked with the synthesized peptide.
Key facts & specifications
Research areas
Antibody Research
Also known as
ARSI; Arylsulfatase I; ASI
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized peptide derived from human ARSI. AA range:311-360
Observed band
64kD
Cellular localization
Secreted . Endoplasmic reticulum . Localized in the intracellular granular structures.
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 311-360
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Monkey
Identifiers & database references
Supplier SKU / catalogue number
ES1711
UniProt entry
Q5FYB1
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00