Trk A (Phospho-Y496) Rabbit pAb

Trk A (Phospho-Y496) Rabbit pAb

Trk A (Phospho-Y496) Rabbit pAb
Manufactured by:ELK Biotechnology
RUO

Trk A (Phospho-Y496) Rabbit pAb

Trk A (Phospho-Y496) Rabbit pAb from ELK Biotechnology

SKU:
ABM104045
Product type
Primary Antibody
TargetTrk A
ApplicationsWB, IF, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemIsoform TrkA-I is found in most non-neuronal tissues. Isoform TrkA-II is primarily expressed in neuronal cells. TrkA-III is specifically expressed by pluripotent neural stem and neural crest progenitors.
PurityThe antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Trk A (Phospho-Y496) Rabbit pAb is a research-use antibody from ELK Biotechnology for studying Trk A in WB, IF, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

This gene encodes a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. The presence of this kinase leads to cell differentiation and may play a role in specifying sensory neuron subtypes. Mutations in this gene have been associated with congenital insensitivity to pain, anhidrosis, self-mutilating behavior, mental retardation and cancer. Alternate transcriptional splice variants of this gene have been found, but only three have been characterized to date. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity This antibody detects endogenous pospho levels of human Trk A (Phospho-Y496)
Validation evidence captions Western blot analysis of various lysates, primary antibody was diluted at 1:1000, 4° over night, secondary antibody(cat: RS23920)was diluted at 1:10000, 37° 1hour.
Key facts & specifications
Research areas
>>MAPK signaling pathway, >>Ras signaling pathway, >>Calcium signaling pathway, >>PI3K-Akt signaling pathway, >>Apoptosis, >>Neurotrophin signaling pathway, >>Inflammatory mediator regulation of TRP channels, >>Pathways in cancer, >>Transcriptional misregulation in cancer, >>Thyroid cancer, >>Central carbon metabolism in cancer
Also known as
High affinity nerve growth factor receptor (EC 2.7.10.1;Neurotrophic tyrosine kinase receptor type 1;TRK1-transforming tyrosine kinase protein;Tropomyosin-related kinase A;Tyrosine kinase receptor;Tyrosine kinase receptor A;Trk-A;gp140trk;p140-TrkA)
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000; IF/ICC 1:100-500; ELISA 1:5000-20000
Immunogen
Synthesized pospho peptide derived from human Trk A (Phospho-Y496)
Observed band
180kD
Cellular localization
Cell membrane ; Single-pass type I membrane protein . Early endosome membrane ; Single-pass type I membrane protein . Late endosome membrane ; Single-pass type I membrane protein . Recycling endosome membrane ; Single-pass type I membrane protein . Rapidly internalized after NGF binding (PubMed:1281417). Internalized to endosomes upon binding of NGF or NTF3 and further transported to the cell body via a retrograde axonal transport. Localized at cell membrane and early endosomes before nerve growth factor (NGF) stimulation. Recruited to late endosomes after NGF stimulation. Colocalized with RAPGEF2 at late endosomes. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000; IF/ICC 1:100-500; ELISA 1:5000-20000
Purification
The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES12561
UniProt entry
P04629
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00