Manufactured by:ELK Biotechnology
RUO

Histone H2B (Acetyl Lys126) rabbit pAb

Histone H2B (Acetyl Lys126) rabbit pAb from ELK Biotechnology

SKU:
ABM103043
Product type
Primary Antibody
TargetHistone H2B
ApplicationsWB, ELISA
Species / reactivityHuman, Mouse, Rat
Expression systemMainly expressed in testis, and the corresponding protein is also present in mature sperm (at protein level). Also found in some fat cells.
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Histone H2B (Acetyl Lys126) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Histone H2B in WB, ELISA workflows with listed reactivity for Human, Mouse, Rat. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a testis/sperm-specific member of the histone H2B family. Transcripts from this gene contain a palindromic termination element. [provided by RefSeq, Aug 2015],

Additional antibody information

Specificity Acetyl-Histone H2B (K126) Polyclonal Antibody detects endogenous levels of Histone H2B protein only when acetylated at K126.
Validation evidence captions Western Blot analysis of A549 cells using Acetyl-Histone H2B (K126) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western Blot analysis of A549 cells using Acetyl-Histone H2B (K126) Polyclonal Antibody. Secondary antibody(catalog#:RS0002) was diluted at 1:20000 | Western blot analysis of lysate from A549 cells, using H2B (Acetyl-Lys126) Antibody.
Key facts & specifications
Research areas
>>Neutrophil extracellular trap formation, >>Alcoholism, >>Viral carcinogenesis, >>Systemic lupus erythematosus
Also known as
HIST1H2BA; TSH2B; Histone H2B type 1-A; Histone H2B, testis; Testis-specific histone H2B; HIST1H2BB; H2BFF; Histone H2B type 1-B; Histone H2B.1; Histone H2B.f; H2B/f; HIST1H2BC; H2BFL; HIST1H2BE; H2BFH; HIST1H2BF;H2BFG; HIST1H2BG; H2BFA; HIST1H2BI; H2BFK; Histone H2B type 1-C/E/F/G/I; Histone H2B.1 A; Histone H2B.a; H2B/a; Histone H2B.g; H2B/g; Histone H2B.h; H2B/h; Histone H2B.k; H2B/k; Histone H2B.l; H2B/l;H2BK126AC
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Immunogen
The antiserum was produced against synthesized Acetyl-peptide derived from human H2B around the Acetylation site of Lys126. AA range:78-127
Observed band
15kD
Cellular localization
Nucleus . Chromosome .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 78-127
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Mouse
Rat
Identifiers & database references
Supplier SKU / catalogue number
ES1102
UniProt entry
Q96A08
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00