Manufactured by:ELK Biotechnology
RUO

ACTG rabbit pAb

ACTG rabbit pAb from ELK Biotechnology

SKU:
ABM102944
Product type
Primary Antibody
TargetACTG
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemB-cell,B-cell lymphoma,Brain,Cajal-Retzius cell,Eye,Hepatocellular carcinoma,Lung,Muscle,No
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

ACTG rabbit pAb is a research-use antibody from ELK Biotechnology for studying ACTG in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2011],

Additional antibody information

Specificity ACTG Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from KB cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Rap1 signaling pathway, >>Phagosome, >>Apoptosis, >>Hippo signaling pathway, >>Focal adhesion, >>Adherens junction, >>Tight junction, >>Platelet activation, >>Neutrophil extracellular trap formation, >>Leukocyte transendothelial migration, >>Thermogenesis, >>Regulation of actin cytoskeleton, >>Thyroid hormone signaling pathway, >>Oxytocin signaling pathway, >>Gastric acid secretion, >>Amyotrophic lateral sclerosis, >>Bacterial invasion of epithelial cells, >>Vibrio cholerae infection, >>Pathogenic Escherichia coli infection, >>Shigellosis, >>Salmonella infection, >>Yersinia infection, >>Influenza A, >>Proteoglycans in cancer, >>Hepatocellular carcinoma, >>Hypertrophic cardiomyopathy, >>Arrhythmogenic right ventricular cardiomyopathy, >>Dilated cardiomyopathy, >>Viral myocarditis, >>Fluid shear stress and atherosclerosis
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Immunogen
Synthesized peptide derived from part region of human protein
Observed band
41kD
Cellular localization
Cytoplasm, cytoskeleton .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES10854
UniProt entry
P63261
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

ELK Biotechnology
£220.00