Manufactured by:ELK Biotechnology
RUO

ATP4B rabbit pAb

ATP4B rabbit pAb from ELK Biotechnology

SKU:
ABM102493
Product type
Primary Antibody
TargetATP4B
ApplicationsWB, ELISA
Species / reactivityHuman, Rat, Mouse
Expression systemColon,Liver,
PurityThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

ATP4B rabbit pAb is a research-use antibody from ELK Biotechnology for studying ATP4B in WB, ELISA workflows with listed reactivity for Human, Rat, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.

Background

The protein encoded by this gene belongs to a family of P-type cation-transporting ATPases. The gastric H+, K+-ATPase is a heterodimer consisting of a high molecular weight catalytic alpha subunit and a smaller but heavily glycosylated beta subunit. This enzyme is a proton pump that catalyzes the hydrolysis of ATP coupled with the exchange of H(+) and K(+) ions across the plasma membrane. It is also responsible for gastric acid secretion. This gene encodes the beta subunit of the gastric H+, K+-ATPase. [provided by RefSeq, Jul 2008],

Additional antibody information

Specificity ATP4B Polyclonal Antibody detects endogenous levels of protein.
Validation evidence captions Western blot analysis of lysates from PC12 cells, primary antibody was diluted at 1:1000, 4°over night
Key facts & specifications
Research areas
>>Oxidative phosphorylation, >>Metabolic pathways, >>Collecting duct acid secretion, >>Gastric acid secretion
Host species
Rabbit
Clonality
Polyclonal
Isotype
IgG
Dilution range
WB 1:500-2000 ELISA 1:5000-20000
Immunogen
Synthesized peptide derived from human protein . at AA range: 160-240
Observed band
32kD
Cellular localization
Apical cell membrane ; Single-pass type II membrane protein . Cell membrane ; Single-pass type II membrane protein . Localized in the apical canalicular membrane of parietal cells. .
Assay type
Antibody validation/application
Source organism
Rabbit
Concentration
1 mg/ml
Working concentration
WB 1:500-2000 ELISA 1:5000-20000
Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Form / buffer
Liquid
Sequence fragment
AA range: 160-240
Quantity per pack
50µL/100µL
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Application validation matrix

Evidence status by application and model.

SpeciesWBIHCIFELISAIPFCMChIP
Human
Rat
Mouse
Identifiers & database references
Supplier SKU / catalogue number
ES10039
UniProt entry
P51164
Storage, stability & shipping
Storage conditions
'-20°C/1 year
Long-term storage
'-20°C/1 year
Storage buffer
Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Datasheet
Open datasheet
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

ATP4BATPase H+/K+ transporting subunit beta

View target guide

ATP4B identifies ATPase H+/K+ transporting subunit beta in Homo sapiens (Human). This target record connects NCBI Gene 496, UniProtKB P51164 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
496
UniProtKB
P51164
ELK Biotechnology
£220.00