Rat ATF4(Activating Transcription Factor 4) ELISA Kit from ELK Biotechnology
Know who made it. See the evidence. Buy through one marketplace.
Rat ATF4(Activating Transcription Factor 4) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring ATF4 in Tissue homogenates, Cell lysates, Other biological fluids samples from Rat. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ATF4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ATF4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ATF4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ATF4 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include TAXREB67; TXREB; CREB-2; DNA-binding protein TAXREB67; Cyclic AMP-responsive element-binding protein 2; Tax-responsive enhancer element-binding protein 67.
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Tissue homogenates | Cell lysates | Other biological fluids |
|---|---|---|---|
| Sample compatibility |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
Activating transcription factor 4 (ATF4), also known as CREB-2 and cyclic AMP-dependent transcription factor ATF-4, is a bZIP transcription factor central to the integrated stress response. Human ATF4 maps to NCBI Gene 468 and reviewed UniProtKB P18848; this record links the verified target to related ABMIUM catalogue products.
Biological function: ATF4 binds cAMP response elements and regulates metabolic and redox programmes while acting as a major transcriptional effector of the integrated stress response. Its translation rises during stresses including endoplasmic-reticulum stress, amino-acid starvation and oxidative stress, enabling adaptive stress-responsive gene expression.
