Human ATP2A2(ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2) ELISA Kit from ELK Biotechnology
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Human ATP2A2(ATPase, Ca++ Transporting, Cardiac Muscle, Slow Twitch 2) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring ATP2A2 in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ATP2A2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ATP2A2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ATP2A2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ATP2A2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include ATP2B; DAR; SERCA2; Calcium pump 2; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; Calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle.
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Serum | Plasma | Tissue homogenates | Cell lysates | Cell culture supernatants | Other biological fluids |
|---|---|---|---|---|---|---|
| Sample compatibility |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
ATP2A2 encodes SERCA2, a sarcoplasmic/endoplasmic-reticulum calcium pump that uses ATP hydrolysis to move cytosolic calcium into intracellular stores. This target record connects the human ATP2A2/SERCA2 reference identifiers with ABMIUM catalogue products targeting SERCA2.
Biological function: ATP2A2/SERCA2 is a magnesium-dependent calcium-transporting ATPase. It couples ATP hydrolysis to movement of Ca2+ from the cytosol into the sarcoplasmic or endoplasmic reticulum lumen, helping maintain intracellular calcium stores and calcium homeostasis. The reviewed UniProt record also supports roles in starvation-induced autophagy and regulation of endoplasmic-reticulum contact sites.
