Mouse S100B(S100 Calcium Binding Protein B) Microsample ELISA Kit from ELK Biotechnology
Know who made it. See the evidence. Buy through one marketplace.
Mouse S100B(S100 Calcium Binding Protein B) Microsample ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring S100B in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Mouse. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse S100B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse S100B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse S100B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse S100B in the samples is then determined by comparing the OD of the samples to the standard curve.
Alternative names include S100-B; NEF; S100Beta; S-100 protein subunit beta; S100 protein beta chain; Soluble protein-100B; Soluble protein 100B.
Fully tested in-house by ABMIUM. Highest confidence. Non-conformities fully supported under the ABMIUM Product Promise.
Quality verified. Independent collaborator or expected-performance data available. Product meets ABMIUM quality standards.
Expected to work based on structural and biochemical data. Not yet directly tested by ABMIUM or a collaborator. No non-conformity support for this combination.
Not recommended for this application or species/sample combination. This may indicate either evidence of unsuitability or that the combination has not been tested and therefore cannot currently be recommended.
Sample compatibility evidence using the same validation key.
| Sample type | Serum | Plasma | Tissue homogenates | Cell lysates | Cell culture supernatants | Other biological fluids |
|---|---|---|---|---|---|---|
| Sample compatibility |
This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
S100B identifies S100 calcium binding protein B in Homo sapiens (Human). This target record connects NCBI Gene 6285, UniProtKB P04271 and related ABMIUM catalogue products.
Biological function: S100B is a calcium-binding member of the S100 protein family with two EF-hand motifs. It participates in calcium-dependent regulation of cellular processes including cell-cycle progression and differentiation and has established roles in neural and glial biology.
