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Calibrated Color Prestained Protein Marker (10-250kDa) from EnkiLife is intended for research workflows. Review the listed specifications and supporting documents to confirm compatibility with the intended method.
This product is a high-purity recombinant color prestained protein marker specifically designed for research experiments. Through scientific proportioning of 11 precise molecular weight proteins (10, 15, 20, 25, 30, 40, 50, 72, 100, 130, 250kDa), it addresses the core pain points of traditional prestained markers "low accuracy and poor stability" providing key support for improved experimental efficiency:
Color differentiation design: 72kDa (orange-red), 25kDa (green), others (blue), allowing real-time observation without staining, no need to pause electrophoresis to judge progress, quick confirmation of transfer efficiency after membrane transfer
Authoritative calibration guarantee: Calibrated with imported brand non-prestained marker standards, molecular weight deviation is far below industry average, accuracy comparable to non-prestained markers
Pre-mixed 1× SDS-PAGE loading buffer, no boiling required
Broad molecular weight coverage: 10-250kDa covers commonly used protein molecular weight ranges in research
Clear band performance: 3-5μL loading volume produces sharp bands, no diffusion or trailing after electrophoresis
| Product Name | Specification 1 (100μL) | Specification 2 (250μL×2) | Specification 3 (250μL×10) |
|---|---|---|---|
| Calibrated Color Prestained Protein Marker (10-250kDa) | Suitable for small batch pre-experiments (approx. 20-30 uses) | Suitable for regular laboratories (approx. 100-150 uses) | Suitable for large laboratories/high-throughput experiments (approx. 500-800 uses) |
| Molecular Weight Node | This Product Deviation | International T Brand Deviation |
|---|---|---|
| 250kDa | ＜2% | 16%（250→210kDa） |
| 130kDa | 6.1%（130→122kDa） |
9.2%（130→118kDa） |
| 100kDa | 5%（100→95kDa） | 15%（100→85kDa） |
| 72kDa&70kDa | 11.1%（72→64kDa） | 5.7%（70→66kDa） |
| 50kDa&55kDa | ＜2%（50kDa） | 9.1%（55→50kDa） |
| 30kDa&35kDa | 3.3%（30-29kDa） | 14.2%（35→30kDa） |
| 25kDa | 8%（25→23kDa） | 4%（25→24kDa） |
2. Buffer System Compatibility: Stable Migration Across Scenarios
Validated across multiple systems including Tris-Glycine buffer (conventional SDS-PAGE system), Tris-Tricine buffer (small molecule protein separation system), and Bis-Tris buffer (high-resolution separation system), this product maintains stable migration in all systems
3. Extreme Condition Stability: Handling Complex Experimental Environments
4. Operational Convenience:
Remove product from -20℃ freezer, place in 4℃ refrigerator (10-20 minutes) or room temperature (5-8 minutes) for natural thawing, strictly avoid water bath heating or boiling;
After thawing, gently mix (recommend centrifugation at 4000-8000rpm for 30s before mixing);
Aspirate 3-5μL, slowly add to gel loading well, adjust loading volume appropriately according to gel properties and experimental habits;
Return unused marker to -20℃, recommend aliquoting into 10-50μL/tube.
Precautions
This product already contains loading buffer, do not heat or boil;
To reduce marker degradation and contamination risks, recommend aliquoting and storing at -20℃;
For research use only.
This product is manufactured by EnkiLife and sold through ABMIUM without relabelling.
ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.
Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.
Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.
