Manufactured by:ELK Biotechnology
RUO

Human DKK1(Dickkopf Related Protein 1) ELISA Kit

Human DKK1(Dickkopf Related Protein 1) ELISA Kit from ELK Biotechnology

SKU:
ABM58802
Product type
ELISA Kits
TargetDKK1
ApplicationsELISA
Species / reactivityHuman
Sample typeSerum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids

Manufacturer provenance

Manufactured by:
ELK Biotechnology
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human DKK1(Dickkopf Related Protein 1) ELISA Kit is a research-use ELISA kit from ELK Biotechnology for measuring DKK1 in Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatant, Other biological fluids samples from Human. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Test principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human DKK1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human DKK1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human DKK1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human DKK1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Target background

Alternative names include SK; Dickkopf Homolog 1(Xenopus Laevis).

Key facts & specifications
Research areas
Signal transduction, Tumor immunity, Hematology, Developmental science, Bone metabolism
Also known as
SK; Dickkopf Homolog 1(Xenopus Laevis)
Assay type
Sandwich
Detection method
Colorimetric (TMB/HRP)
Sensitivity
0.058 ng/mL
Detection range
0.16-10 ng/mL
Plate format
96-well pre-coated
Form / buffer
Pre-coated microplate kit
Quantity per pack
48T
Validation & performance evidence
ABMIUM Validated™ ABMIUM Verified™ Expected performance Not suitable

Sample type validation

Sample compatibility evidence using the same validation key.

Sample typeSerumPlasmaTissue homogenatesCell lysatesCell culture supernatantsOther biological fluids
Sample compatibility
Identifiers & database references
Supplier SKU / catalogue number
ELK2097
UniProt entry
O94907
Storage, stability & shipping
Storage conditions
Store according to the conditions stated in the product manual.
Long-term storage
Store according to the conditions stated in the product manual.
Compliance & documents
CE marked
No
ISO certifications
ISO 9001
Product manual
Open manual
Frequently asked questions
Who manufactured this product?

This product is manufactured by ELK Biotechnology and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

DKK1dickkopf Wnt signaling pathway inhibitor 1

View target guide

DKK1 identifies dickkopf Wnt signaling pathway inhibitor 1 in Homo sapiens (Human). This target record connects NCBI Gene 22943, UniProtKB O94907 and related ABMIUM catalogue products.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
22943
UniProtKB
O94907
ELK Biotechnology
£284.00