Rat LEP(Leptin) ELISA Kit

Rat LEP(Leptin) ELISA Kit - ABMIUM

Rat LEP(Leptin) ELISA Kit - ABMIUM
Manufactured by:Elabscience
Save 30%ABMIUM Verified™RUO

Rat LEP(Leptin) ELISA Kit

Rat LEP(Leptin) ELISA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM12453-1
Product type
ELISA Kits
TargetLEP

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

Know who made it. See the evidence. Buy through one marketplace.

Product description

Rat LEP(Leptin) ELISA Kit is a research-use ELISA kit from Elabscience for measuring LEP. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Key facts & specifications
Research areas
Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction, Stem cells
Also known as
Lep,Ob,Leptin,Obesity factor
Cross-reactivity
This kit recognizes Rat LEP in samples. No significant cross-reactivity or interference between Rat LEP and analogues was observed
Incubation temperature
2-8℃
Assay type
Traditional ELISA Kits
Detection method
Colorimetric method,ELISA,Sandwich
Plate format
48-well microplate plates pre-coated with anti-LEP capture antibody
Technical note
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat LEP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat LEP. You can calculate the concentration of Rat LEP in the samples by comparing the OD of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-EL-R0582_48T
Gene symbol
LEP
Gene ID
25608
UniProt accession
P50596
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

LEPleptin

View target guide

LEP identifies leptin in Homo sapiens (Human). This target record connects NCBI Gene 3952, UniProtKB P41159 and related ABMIUM catalogue products.

Biological function: LEP (leptin) is an adipocyte-derived hormone that signals through the leptin receptor to regulate energy balance, appetite and neuroendocrine responses. It also influences metabolic, reproductive and immune physiology.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
3952
UniProtKB
P41159
Browse all LEP productsRead target context & evidence
Elabscience
£295.00£206.50