Manufactured by:Elabscience
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Human CA9(Carbonic Anhydrase Ⅸ) ELISA Kit

Human CA9(Carbonic Anhydrase Ⅸ) ELISA Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM11483-1
Product type
ELISA Kits
TargetCA9

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Human CA9(Carbonic Anhydrase Ⅸ) ELISA Kit is a research-use ELISA kit from Elabscience for measuring CA9. Use the listed assay range, sample guidance and protocol documents to assess suitability for the intended study.

Key facts & specifications
Research areas
Cancer, Cardiovascular, Metabolism, Signal transduction
Also known as
CAIX,CA-IX,MN,Carbonic Dehydratase,RCC-Associated Protein G250,
Cross-reactivity
This kit recognizes Human CA9 in samples. No significant cross-reactivity or interference between Human CA9 and analogues was observed
Incubation temperature
2-8℃
Assay type
Traditional ELISA Kits
Detection method
Colorimetric method,ELISA,Sandwich
Plate format
48-well microplate plates pre-coated with anti-CA9 capture antibody
Technical note
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CA9. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CA9 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CA9, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CA9. You can calculate the concentration of Human CA9 in the samples by comparing the OD of the samples to the standard curve.
Identifiers & database references
Supplier SKU / catalogue number
E-EL-H0015_48T
Gene symbol
CA9
Gene ID
768
UniProt accession
Q16790
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Research target context

CA9carbonic anhydrase 9

View target guide

Carbonic anhydrase 9 (CA9), also called carbonic anhydrase IX or CAIX, is a transmembrane zinc metalloenzyme involved in carbon dioxide/bicarbonate interconversion and pH regulation. ABMIUM catalogue products linked below support research on CA9 biology.

Biological function: Carbonic anhydrase 9 (CA9/CA IX/CAIX) is a transmembrane zinc-dependent carbonic anhydrase that catalyses the reversible interconversion of carbon dioxide and water with bicarbonate and hydrogen ions. Its expression is induced by hypoxia, linking CA9 to cellular acid-base regulation and hypoxia-associated research.

Organism
Multiple species; representative: Homo sapiens (Human)
NCBI Gene
768
UniProtKB
Q16790
Elabscience
£363.00£254.10