Control: Normal Hela cells were not treated with DMSO. 10% DMSO-4h: Hela cells were treated with 10% DMSO for 4h. HeLa cells stained with Caspase 3/7 Substrates (green) and DAPI Reagent (25 μg/mL) (blue).
Manufactured by:Elabscience
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Caspase 3/7 and DAPI Double Staining Kit

Caspase 3/7 and DAPI Double Staining Kit with collaborator-tested sample matrix evidence from Elabscience.

SKU:
ABM10809-1
Product type
Assay Kits

Manufacturer provenance

Manufactured by:
Elabscience
Sold by:
ABMIUM
Fulfilled from:
Manufacturer facility or confirmed fulfilment location

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Product description

Caspase 3/7 and DAPI Double Staining Kit from Elabscience is intended for research workflows. Review the listed specifications and supporting documents to confirm compatibility with the intended method.

Key facts & specifications
Incubation temperature
This product can be stored at 2~8℃ for 1 year in the dark.
Assay type
Assay Kits
Detection method
Fluorometric method,Flow cytometry
Technical note
The Caspase 3/7 Substrates（Green）are based on novel fluorogenic DNA dyes that have been coupled to the caspase 3/7 recognition sequence (DEVD),which is both non-fluorescent and nonfunctional as a DNA dye.When it rapidly crosses cell membranes to enter the cytoplasm and cleaved by caspase 3/7 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the Caspase 3/7 substrates allow detection of caspase 3/7 activity and visualization of apoptotic nuclear morphology simultaneously. Due to the loss of integrity of membrane, DAPI can enter late apoptotic or necrotic cells to stain DNA. DAPI co-staining of cells with Caspase 3/7 Substrates allows simultaneous differentiation of late-apoptotic cells, necrotic cells, and caspase 3/7 enzyme activity during different apoptotic periods.
Identifiers & database references
Supplier SKU / catalogue number
E-CK-A833_100Assays
Storage, stability & shipping
Shelf life
12 months from shipping dates
Shipped at
Wet ice (2-8 degrees C)
Short-term storage
4 degrees C for up to 6 months; do not freeze pre-coated plate
Long-term storage
4 degrees C for up to 12 months
Aliquoting guidance
Lyophilised standards and detection antibody may be reconstituted and aliquoted; store reconstituted aliquots at -20 degrees C for up to 2 weeks
Compliance & documents
REACH compliant
Yes
ISO certifications
ISO 9001:2015, ISO 14001:2015, ISO 45001:2018
Datasheet
Open datasheet
Product manual
Open manual
Safety data sheet
Open SDS
Frequently asked questions
Who manufactured this product?

This product is manufactured by Elabscience and sold through ABMIUM without relabelling.

Can ABMIUM ship this product internationally?

ABMIUM supports global orders. Availability, shipping requirements and applicable import arrangements are confirmed before fulfilment where required.

Can I check suitability before ordering?

Yes. Send your target, sample, application and experimental conditions through ABMIUM MATCH or technical support.

Is this product for clinical use?

Unless the listing expressly states otherwise, ABMIUM products are supplied for research use only and are not for diagnostic or therapeutic use.

Elabscience
£290.00£232.00