ABM-16361
CollaboratorElabscience
Target
TNFR1/TNFRSF1APurity
>92% by SDS-PAGEMol. weight
22.7 kDaActivity
1. Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) ...Species
Human
1. Immobilized human TNFRI-His at 10 μg/mL (100 μl/well) can bind biotinylated human TNFα. The EC50 of biotinylated human TNFα is 5-12 μg/mL. 2. Measured by its ability to inhibit TNFα-mediated cytotoxicity in L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-1μg/mL in the presence of 0.25 ng/mL recombinant human TNFα.HEK293 Cells< 1.0 EU per μg of the protein as determined by the LAL method.