DNA and RNA FISH Application Guide

How DNA and RNA FISH works

FISH uses a labelled nucleic-acid probe that hybridises to a complementary target sequence. Sample preparation, probe design, denaturation or permeabilisation, hybridisation stringency and image acquisition all influence signal quality.

1. Define the genomic or RNA target and choose a compatible probe.
2. Prepare and preserve the cells or tissue while maintaining target accessibility.
3. Hybridise under conditions appropriate to the probe and sample.
4. Wash, counterstain, image and interpret the signal using suitable controls.

Experimental planning and controls

Match the probe chemistry to the sample type and target abundance. Include appropriate positive, negative and background controls and confirm whether the selected protocol is designed for DNA, RNA, metaphase chromosomes, fixed cells or tissue. Product-specific instructions take priority over a general workflow.