Forside » ELISA Data Analyser

Scientific analysis tool · Research Use Only

ELISA Data Analyser

Analyse 96-well ELISA absorbance data, build a standard curve, review duplicate precision and CV values, estimate sample concentrations and export a clear record of the run.

Quick start 1 Enter your standards or load the demo dataset. 2 Map the 8 × 12 plate and enter or paste OD values. 3 Review the curve, replicate CV and calculated results before export.
ELISA Analyser Sandwich
Assay settings
Settings stay visible while you work.
Assay Information
Assay / Project Name
Analyst Name
Run Date
Notes
Assay Configuration
ELISA Type
Concentration Units
Acceptable CV% Threshold
Chart Appearance
Fitted curve colour
Standard point colour
Point size - 5px
Show grid lines
Show equation on chart
Data Management

Use the demo dataset to explore the workflow, or clear the current run before entering new assay data.

Standard Series
Enter known concentrations & duplicate OD readings
IDConcOD Rep 1OD Rep 2Avg ODCorr. OD
Standard Curve
x = Corrected OD · y = Concentration
Fitted curveStandards
No curve data
Enter at least 3 standards with OD values
96-Well Plate Editor
Click a well · Tab/Enter to advance · Arrow keys to navigate
Assigning sample names
Standard
Blank
Sample
Core Definitions
Corrected OD
Sandwich/Direct/Indirect: Avg OD − Blank Avg OD. Competitive: Raw Avg OD.
Standard Curve
Review the fitted relationship and R² before accepting calculated concentrations.
CV%
SD of replicate concentrations ÷ mean × 100. Review against the threshold set above.
OOR
Sample OD outside the standard curve range. Re-assay at a suitable dilution.
ELISA Format Comparison
FormatSignal vs ConcBlank Correction
SandwichIncreases ↑Subtract blank OD
DirectIncreases ↑Subtract blank OD
IndirectIncreases ↑Subtract blank OD
CompetitiveDecreases ↓Raw OD

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