Bmx-Kaninchen-pAk von ELK Biotechnology Read more ›
Bmx rabbit pAb is a research-use antibody from ELK Biotechnology for studying ETK in WB, IHC, IF, ELISA workflows with listed reactivity for Human, Mouse. Specifications and supporting documents below should be reviewed when planning the experiment.
This gene encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain, which mediates membrane targeting by binding to phosphatidylinositol 3,4,5-triphosphate (PIP3), and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway, and regulates differentiation and tumorigenicity of several types of cancer cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016],
| Specificity | Bmx Polyclonal Antibody detects endogenous levels of Bmx protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Bmx Polyclonal Antibody diluted at 1:1000 | Immunofluorescence analysis of A549 cells, using ETK Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using ETK Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from A549 cells, using ETK Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1783 |
| Target | ETK |
| Research areas | Antibody Research |
| Reactivity | Mensch;Maus |
| Applications | WB,IHC,IF,ELISA |
| Conjugation | Unkonjugiert |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunhistochemie: 1/100 - 1/300. Immunfluoreszenz: 1/200 - 1/1000. ELISA: 1/10000. Noch nicht in anderen Anwendungen getestet. |
| Host | Kaninchen |
| Clonality | Polyklonal |
| Isotype | IgG |
| Immunogen | Das Antiserum wurde gegen synthetisiertes Peptid hergestellt, das aus humanem C-terminalem ETK gewonnen wurde. AA-Bereich: 532-581 |
| Concentration | 1 mg/ml |
| Purification | Der Antikörper wurde mittels Affinitätschromatographie unter Verwendung eines Epitop-spezifischen Immunogens aus Kaninchenantiserum affinitätsgereinigt. |
| Assay type | Antikörpervalidierung/-anwendung |
| Expression system | Hoch exprimiert in Zellen mit großem Migrationspotential, einschließlich Endothelzellen und metastatischen Karzinomzelllinien. |
| Purity | Der Antikörper wurde mittels Affinitätschromatographie unter Verwendung eines Epitop-spezifischen Immunogens aus Kaninchenantiserum affinitätsgereinigt. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Mensch | |||||||
| Maus |
| Species | Dilution | Notes |
|---|---|---|
| Mensch | Western Blot: 1/500 - 1/2000. Immunhistochemie: 1/100 - 1/300. Immunfluoreszenz: 1/200 - 1/1000. ELISA: 1/10000. Noch nicht in anderen Anwendungen getestet. | — |
| Maus | — | — |
| Species | Dilution | Notes |
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Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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