Bmx rabbit pAb
Bmx-Kaninchen-pAk von ELK Biotechnology Read more ›

Product Description
Bmx rabbit pAb is an ELK Biotechnology antibody for research workflows involving ETK.
Background
This gene encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain, which mediates membrane targeting by binding to phosphatidylinositol 3,4,5-triphosphate (PIP3), and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway, and regulates differentiation and tumorigenicity of several types of cancer cells. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Mar 2016],
Additional antibody information
| Specificity | Bmx Polyclonal Antibody detects endogenous levels of Bmx protein. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Bmx Polyclonal Antibody diluted at 1:1000 | Immunofluorescence analysis of A549 cells, using ETK Antibody. The picture on the right is blocked with the synthesized peptide. | Immunohistochemistry analysis of paraffin-embedded human thyroid gland tissue, using ETK Antibody. The picture on the right is blocked with the synthesized peptide. | Western blot analysis of lysates from A549 cells, using ETK Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | ETK |
| Also known as | BMX; Zytoplasmatische Tyrosinproteinkinase BMX; Knochenmark-Tyrosinkinase-Gen in Chromosom X Protein; Epitheliale und endotheliale Tyrosinkinase; ETK; NTK38 |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. Immunhistochemie: 1/100 - 1/300. Immunfluoreszenz: 1/200 - 1/1000. ELISA: 1/10000. Noch nicht in anderen Anwendungen getestet. |
| Species reactivity | Mensch;Maus |
| Observed band | 78 kD |
| Cellular localization | Zytoplasma. Lokalisiert sich an den Rändern von ausbreitenden Zellen, wenn es mit BCAR1 komplexiert ist. |
| Purity | Der Antikörper wurde mittels Affinitätschromatographie unter Verwendung eines Epitop-spezifischen Immunogens aus Kaninchenantiserum affinitätsgereinigt. |
| Form / buffer | Flüssigkeit in PBS, die 50 % Glycerin, 0,5 % BSA und 0,02 % Natriumazid enthält. |
| Research area | Antikörperforschung |
| Host species | Kaninchen |
| Clonality | Polyklonal |
| Isotype | IgG |
| Conjugation | Unkonjugiert |
| Tested applications |
WBIHCIFELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. Immunhistochemie: 1/100 - 1/300. Immunfluoreszenz: 1/200 - 1/1000. ELISA: 1/10000. Noch nicht in anderen Anwendungen getestet. |
| Immunogen | Das Antiserum wurde gegen synthetisiertes Peptid hergestellt, das aus humanem C-terminalem ETK gewonnen wurde. AA-Bereich: 532-581 |
| Purification | Der Antikörper wurde mittels Affinitätschromatographie unter Verwendung eines Epitop-spezifischen Immunogens aus Kaninchenantiserum affinitätsgereinigt. |
Reactivity & Application Validation
2 artens| Arten | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Mensch | |||||||
| Maus |
| Arten | Dilution | Notes |
|---|---|---|
| Mensch | Western Blot: 1/500 - 1/2000. Immunhistochemie: 1/100 - 1/300. Immunfluoreszenz: 1/200 - 1/1000. ELISA: 1/10000. Noch nicht in anderen Anwendungen getestet. | — |
| Maus | — | — |
| Arten | Dilution | Notes |
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| Arten | Dilution | Notes |
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Spezifikationen
Lagerung und Stabilität
Compliance & Zertifizierungen
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.