Cleaved-Cathepsin L2 (L114) Kaninchen-pAk von ELK Biotechnology Read more ›
Cleaved-Cathepsin L2 (L114) rabbit pAb is a research-use antibody from ELK Biotechnology for studying Cathepsin L2 in WB, ELISA workflows with listed reactivity for Human, Monkey. Specifications and supporting documents below should be reviewed when planning the experiment.
The protein encoded by this gene, a member of the peptidase C1 family, is a lysosomal cysteine proteinase that may play an important role in corneal physiology. This gene is expressed in colorectal and breast carcinomas but not in normal colon, mammary gland, or peritumoral tissues, suggesting a possible role for this gene in tumor processes. Alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jan 2011],
| Specificity | Cleaved-Cathepsin L2 (L114) Polyclonal Antibody detects endogenous levels of fragment of activated Cathepsin L2 protein resulting from cleavage adjacent to L114. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-Cathepsin L2 (L114) Polyclonal Antibody | Western blot analysis of lysates from 293 and COS cells, treated with etoposide 25uM 1h, using CATL2 (Cleaved-Leu114) Antibody. The lane on the right is blocked with the synthesized peptide. |
| Collaborator | ELK Biotechnology |
| Partner model | ES1032 |
| Target | Cathepsin L2 |
| Research areas | >>Lysosome, >>Apoptosis |
| Reactivity | Mensch;Affe |
| Applications | WB,ELISA |
| Conjugation | Unkonjugiert |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Noch nicht in anderen Anwendungen getestet. |
| Host | Kaninchen |
| Clonality | Polyklonal |
| Isotype | IgG |
| Immunogen | Das Antiserum wurde gegen ein synthetisiertes Peptid, das von menschlichem CATL2 abgeleitet ist, hergestellt. AA-Bereich: 95-144 |
| Concentration | 1 mg/ml |
| Purification | Der Antikörper wurde mittels Affinitätschromatographie unter Verwendung eines Epitop-spezifischen Immunogens aus Kaninchenantiserum affinitätsgereinigt. |
| Assay type | Antikörpervalidierung/-anwendung |
| Expression system | Vorwiegend im Thymus und im Hoden exprimiert. Auch im Hornhautepithel und in geringerem Maße im Bindehautepithel und in der Haut exprimiert. |
| Purity | Der Antikörper wurde mittels Affinitätschromatographie unter Verwendung eines Epitop-spezifischen Immunogens aus Kaninchenantiserum affinitätsgereinigt. |
| Species | WB | IHC | IF | ELISA | IP | FCM | ChIP |
|---|---|---|---|---|---|---|---|
| Mensch | |||||||
| Affe |
| Species | Dilution | Notes |
|---|---|---|
| Mensch | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. In anderen Anwendungen noch nicht getestet. | — |
| Affe | — | — |
| Species | Dilution | Notes |
|---|
Collaborator meets ABMIUM quality standards and overall product performance standards have been met.
Quality management certification.
For research applications. Not for diagnostic use.
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