Cleaved-Cathepsin D LC (G65) rabbit pAb
Cleaved-Cathepsin D LC (G65) Kaninchen-pAk von ELK Biotechnology Read more ›

Product Description
Cleaved-Cathepsin D LC (G65) rabbit pAb is an ELK Biotechnology antibody for research workflows involving Cathepsin D.
Background
This gene encodes a member of the A1 family of peptidases. The encoded preproprotein is proteolytically processed to generate multiple protein products. These products include the cathepsin D light and heavy chains, which heterodimerize to form the mature enzyme. This enzyme exhibits pepsin-like activity and plays a role in protein turnover and in the proteolytic activation of hormones and growth factors. Mutations in this gene play a causal role in neuronal ceroid lipofuscinosis-10 and may be involved in the pathogenesis of several other diseases, including breast cancer and possibly Alzheimer's disease. [provided by RefSeq, Nov 2015],
Additional antibody information
| Specificity | Cleaved-Cathepsin D LC (G65) Polyclonal Antibody detects endogenous levels of fragment of activated Cathepsin D LC protein resulting from cleavage adjacent to G65. |
|---|---|
| Validation evidence captions | Western Blot analysis of various cells using Cleaved-Cathepsin D LC (G65) Polyclonal Antibody | Western blot analysis of lysates from COS7 cells, treated with etoposide 25uM 1h, using CATD (light chain,Cleaved-Gly65) Antibody. The lane on the right is blocked with the synthesized peptide. |
Key Facts
| Target | Katapsin D |
| Also known as | CTSD; CPSD; Kathepsin D |
| Concentration | 1 mg/ml |
| Working concentration | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Noch nicht in anderen Anwendungen getestet. |
| Species reactivity | Mensch;Affe |
| Observed band | 17 kDa |
| Cellular localization | Lysosom. Melanosom. Sekretierter extrazellulärer Raum. Mittels Massenspektrometrie in Melanosomenfraktionen von Stadium I bis Stadium IV identifiziert. In Aortenproben als extrazelluläres Protein nachgewiesen, das locker an die Matrix gebunden ist (PubMed:20551380). . |
| Purity | Der Antikörper wurde mittels Affinitätschromatographie unter Verwendung eines Epitop-spezifischen Immunogens aus Kaninchenantiserum affinitätsgereinigt. |
| Form / buffer | Flüssigkeit in PBS, die 50 % Glycerin, 0,5 % BSA und 0,02 % Natriumazid enthält. |
| Research area | >>Sphingolipid-Signalweg;>>Autophagie - Tier;>>Lysosom;>>Apoptose;>>Östrogen-Signalweg;>>Tuberkulose;>>Diabetische Kardiomyopathie |
| Host species | Kaninchen |
| Clonality | Polyklonal |
| Isotype | IgG |
| Conjugation | Unkonjugiert |
| Tested applications |
WBELISA |
| Dilution range | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Noch nicht in anderen Anwendungen getestet. |
| Immunogen | Das Antiserum wurde gegen synthetisiertes Peptid, das von menschlichem CATD stammt, hergestellt. AA-Bereich: 46-95 |
| Purification | Der Antikörper wurde mittels Affinitätschromatographie unter Verwendung eines Epitop-spezifischen Immunogens aus Kaninchenantiserum affinitätsgereinigt. |
Reactivity & Application Validation
2 artens| Arten | WB | IHC | IF | ELISA | IP | FCM | CHIP |
|---|---|---|---|---|---|---|---|
| Mensch | |||||||
| Affe |
| Arten | Dilution | Notes |
|---|---|---|
| Mensch | Western Blot: 1/500 - 1/2000. ELISA: 1/20000. In anderen Anwendungen noch nicht getestet. | — |
| Affe | — | — |
| Arten | Dilution | Notes |
|---|
Spezifikationen
Lagerung und Stabilität
Compliance & Zertifizierungen
Manufactured under ISO 9001:2015 quality management standards.
Not intended for diagnostic or therapeutic use.